Kalstein

Biosicherheitswerkbank Klasse II A2 YR0090C-SS

Personal · Produkt · Umgebung · dreifacher Schutz mit HEPA-H14-Filtration

Biosicherheitswerkbank Klasse II A2 YR0090C-SS

Die Kalstein YR0090C-SS ist eine Klasse-II-Typ-A2-Biosicherheitswerkbank für mikrobiologische und biomedizinische Arbeiten mit Erregern der Stufen BSL-1 bis BSL-3. HEPA-H14-Filter halten 99,995 % der Partikel ≥ 0,3 µm zurück; 70 % der Luft werden rezirkuliert, 30 % abgeführt.

Von Kalstein · Zertifizierte Laborausstattung

Interactive tour · Explore the YR0090C-SS

Every component, engineered for containment

Six precision-engineered systems working together — press play for a guided tour or click any point on the cabinet to inspect it in detail.

Prüfbare Komponenten

  • Dual HEPA filtrationAir filtration

    Supply and exhaust HEPA filtration retains particles ≥ 0.3 µm for Class II A2 containment.

  • Digital control panelControl

    Fan, UV lamp and LED lighting are operated from one digital interface.

  • UV disinfection lampDecontamination

    Germicidal UV supports chamber decontamination between operations.

  • LED work lightingLighting

    Waterproof LED lamps provide uniform visibility across the work zone.

  • Tempered glass sashSafety

    The front sash works as a physical barrier between operator and samples.

  • AISI 304 work zoneMaterials

    Stainless work surface designed for cleaning and decontamination.

Hauptmerkmale

  • Klasse-II-Typ-A2-Konfiguration zertifiziert für BSL-1 bis BSL-3
  • HEPA-H14 Zu- und Abluftfilter — 99,995 % bei 0,3 µm
  • Abwärts 0,33 m/s und Zuluft 0,55 m/s für stabile laminare Eindämmung
  • Arbeitskammer aus Edelstahl AISI 304 mit abgerundeten Ecken
  • Motorisierte Frontscheibe mit Sicherheitsalarm
  • Mikroprozessor-Steuerung mit Echtzeit-Luftstromüberwachung

Dreifacher Schutz · Ingenieursebenen

Personnel protection

Inflow curtain at 0.55 m/s prevents aerosols from escaping the work zone, isolating the operator from BSL-1 to BSL-3 agents.

  • Inflow barrier
  • Front sash interlock
  • Audible alarms
Inflow velocity
0.55 m/s
Face velocity
≥ 100 fpm
Max biosafety level
BSL-3

Product protection

HEPA-filtered downflow at 0.33 m/s bathes the sample in ISO 5 laminar air, keeping the culture free from environmental contamination.

  • Downflow laminar
  • ISO 5 work zone
  • HEPA H14 downflow
Downflow velocity
0.33 m/s
Work zone class
ISO 5
Retention @ 0.3 µm
99.995%

Environmental protection

30% of the air is exhausted through a second HEPA H14 filter, releasing only clean air back into the laboratory or ducted exhaust.

  • HEPA H14 exhaust
  • Top duct connection
  • Room air quality preserved
Exhaust ratio
30%
Exhaust filtration
HEPA H14
Noise level
≤ 62 dB

Luftstrommodi · was in jede Richtung passiert

  • Downflow — HEPA downflow at 0.33 m/s

    Vertical laminar air descends from the supply HEPA over the entire work zone, keeping the sample under ISO 5 conditions.

    Velocity: 0.33 m/s

  • Inflow — Inflow curtain at 0.55 m/s

    Room air enters through the front grille at 0.55 m/s, creating an air barrier that stops aerosols from escaping.

    Velocity: 0.55 m/s

  • Exhaust — HEPA exhaust through the top duct

    30% of the internal air is HEPA H14 filtered and released through the top duct.

    Ratio: 30%

  • Full cycle — Simultaneous triple protection

    Downflow, inflow and exhaust combine to protect personnel, product and environment at the same time.

    Configuration: Class II A2

Technische Daten

ModellYR0090C-SS
TypClass II Type A2
FiltrationHEPA H14 (99.995% @ 0.3 µm)
Abwärts-Geschwindigkeit0.33 m/s
Zuluft-Geschwindigkeit0.55 m/s
Abluft-Verteilung30% exhaust · 70% recirculation
Außenmaße1350 × 800 × 2250 mm
Arbeitskammer1130 × 590 × 660 mm
Geräuschpegel≤ 62 dB
Beleuchtung≥ 1000 lux
Stromversorgung220 V / 50-60 Hz · 550 W

Anwendungen

  • Mikrobiologie und Zellkultur
  • Virologie und BSL-3-Forschung
  • Klinische Diagnostik und IVF
  • Pharmazeutische Herstellung
  • Molekularbiologie und PCR

Praxisbeispiele · geführte Abläufe

Cell culture and tissue work

Sterile cell culture in a Class II Type A2 environment for cell lines, primary cultures and tissue processing.

Typische Dauer: 30–120 min

  1. Sanitize the work surface with 70% ethanol
  2. Start the cabinet and wait for stable airflow
  3. Transfer materials through the front opening
  4. Work in the front-to-back laminar zone
  5. Decontaminate before shutdown

Molecular biology and PCR setup

Prepare PCR reactions and molecular assays free from cross-contamination.

Typische Dauer: 20–60 min

  1. UV decontaminate for 15 minutes
  2. Load pipettes, tips and reagents
  3. Prepare master mix in the clean zone
  4. Aliquot into reaction tubes
  5. Clean and log the session

BSL-3 pathogen handling

Manipulation of high-risk agents when integrated in a BSL-3 laboratory suite.

Typische Dauer: 45–180 min

  1. Verify certification and airflow reports
  2. Don PPE and follow SOPs
  3. Introduce material via pass-through
  4. Perform the procedure inside the containment
  5. Autoclave waste and decontaminate the chamber

Ideal for applications in

Where the YR0090C-SS performs

Explore each field the YR0090C-SS was engineered for — click any industry to reveal the specific benefits, key metrics and how the cabinet supports the workflow.

Microbiology laboratories

Culture handling, colony isolation and pathogen manipulation under sterile conditions.

key benefits

  • Sterile culture handling
  • Colony isolation & sub-culturing
  • Pathogen manipulation up to BSL-2
Cleanliness
ISO 5
Downflow
0.45 m/s
Continuous
24/7

Biotechnology & research

Cell culture, molecular biology and genetic engineering with sample-to-operator isolation.

key benefits

  • Primary & stable cell lines
  • Transfection / transduction
  • CRISPR & genetic engineering
LED lighting
2×10 W
Stainless
AISI 304
Filtration
Dual HEPA

Pharmaceutical industry

Sterile compounding, formulation R&D and QC of aseptic preparations.

key benefits

  • USP 797 aseptic compounding
  • Chemotherapy admixtures
  • Small-batch sterile filling
Tipo A2
Class II
Recirc / exhaust
70/30
Stainless
AISI 304

Clinical & diagnostic labs

Processing of blood, tissue and clinical specimens with biological containment.

key benefits

  • Blood & serum processing
  • Tissue & biopsy handling
  • Molecular diagnostics prep
Containment
BSL-2
Decontamination
UV lamp
@ 0.3 µm
≥99.999%

Biohazardous materials

Safe manipulation of BSL-2 agents, low-volatility chemicals and infectious samples.

key benefits

  • BSL-2 infectious agents
  • Trace volatile chemicals
  • Radionuclide trace work
HEPA exhaust
30%
Tempered glass
5 mm
Plenum
Sealed

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Häufig gestellte Fragen

Welche Biosicherheitsstufe?

Zertifiziert für BSL-1, BSL-2 und BSL-3 bei Installation und Validierung nach EN 12469 / NSF 49.

Klasse II Typ A2?

Ja. 70 % HEPA-rezirkuliert, 30 % HEPA-abgeführt über den oberen Kanal.

Filtration?

HEPA-H14 in Zu- und Abluft, Rückhalt ≥ 99,995 % bei 0,3 µm.

Vor-Ort-Validierung?

Ja. Kalstein bietet Inbetriebnahme, Partikelzählung und Luftstromvalidierung nach EN 12469 und NSF/ANSI 49.